منابع مشابه
Cross-linked Polyampholytes
In connection with an investigation of the staining of intracellular antigens with ferritin-antibody conjugates (1), we required an embedding material which was extensively ionic. Although several hydrophilic embedding materials have been described (cf. reference 2), to our knowledge no satisfactory ionic ones have been reported. We sought to prepare an ionic embedding material by the bulk poly...
متن کاملInstabilities of charged polyampholytes.
We consider polymers formed from a (quenched) random sequence of charged monomers of opposite signs. Such polymers, known as polyampholytes (PAs), are compact when completely neutral and expanded when highly charged. We examine the transition between the two regimes by Monte Carlo simulations, and by analogies to charged drops. We find that the overall excess charge, Q, is the main determinant ...
متن کاملExcess Charge in Polyampholytes
Polyampholytes (PAs) are polymers composed of (quenched) random sequences of positive and negatively charged monomeric groups. We show that the radius of gyration, Rg, of a PA strongly depends on its overall excess chargeQ, and is very weakly influenced by other aspects of the sequence. For Q < Qc ≈ q0 √ N , where N is the number of monomers of charge ±q0, the PA is compact, while for Q > Qc it...
متن کاملShape Phase Transition of Polyampholytes
We have studied the transition in shape of two dimensional polyampholytes using Monte Carlo simulation. We observe that polymers with randomly charged monomers get into a globular shape at lower temperatures, provided that their total charge is below a critical value Q c. Collapse into globular form happens for all forms of force law, but the critical charge depends on the form of the force law...
متن کاملCross-linked informofers.
The proteins of 30S RNP particles containing pre-mRNA (hnRNA) were cross-linked with bifunctional reagents (dimethyl-suberimidate and dimethyl-3,3'-dithiobispropionimidate). Further treatment with 1 or 2 M NaCl dissociates all RNA from protein. However, a significant part of protein particles--informofers being cross-linked survived high salt treatment. Their sedimentation coefficients were clo...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1964
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.20.3.518